recombinant mouse il 4 (R&D Systems)
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R&D Systems
recombinant mouse il 4
Recombinant Mouse Il 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+4/Mouse+IL-4+MAb+(Clone+30340)/pm41962806-49-40-43
Average 93 stars, based on 58 article reviews
Recombinant Mouse Il 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+4/Mouse+IL-4+MAb+(Clone+30340)/pm41962806-49-40-43
Average 93 stars, based on 58 article reviews
recombinant mouse il 4 - by Bioz Stars,
2026-10
93/100 stars
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Purification:Article Title: The metabolic program of inflammatory eosinophils accounts for chronic parasite-induced skin disease Article Snippet: Slides were kept overnight at R, and images were taken using a Keyence fluorescence microscope (Keyence, BZ-X800/ BZ-X810) with a 40x or 100x objective. .. Naïve CD4 + CD62L + T cells, purified from the spleens of uninfected C57BL/6N mice using the naïve CD4 + T cell isolation kit (Miltenyi Biotec), were stimulated for 3 days with immobilized anti-CD3 (clone 145–2C11, BioXCell; culture wells coated with 5 μg/mL) in the presence of Th1-skewed conditions, i.e. with addition of IL-12 (R&D System, 419-ML, 5 ng/μL), Article Title: Metabolically reprogrammed eosinophils impair T cell immunity and cause chronic skin infection. Article Snippet: Slides were kept overnight at R, and images were taken using a Keyence fluorescence © The Author(s) EMBO Molecular Medicine 15 microscope (Keyence, BZ-X800/ BZ-X810) with a 40x or 100x objective. .. Co-culture of sorted eosinophils with in vitro-generated Th1 or Th2 cells Naive CD4+CD62L+ T cells, purified from the spleens of uninfected C57BL/6N mice using the naive CD4+ T cell isolation kit (Miltenyi Biotec), were stimulated for 3 days with immobilized anti-CD3 (clone 145–2C11, BioXCell; culture wells coated with 5 μg/mL) in the presence of Th1- or Th2-skewed conditions (Th1: addition of IL-12 [R&D System, 419-ML, 5 ng/mL], Article Title: Fludarabine Ameliorates Lupus Nephritis by Inhibiting T Cell Infiltration through STAT1 in R848-induced Mice Models Article Snippet: CD4+ T cells were purified from spleens of C57BL/6 mice using the CD4+ T cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions. .. For Th1 cell differentiation, purified CD4+ T cells were seeded at 1×106 cells/well and were activated with mouse T-activator CD3/CD28 DynabeadsTM (Invitrogen) and treated with 10 ng/mL IL-2, 10 ng/mL IL-12, 5 ng/mL IFNγ, 10 μg/mL Article Title: Metabolically reprogrammed eosinophils impair T cell immunity and cause chronic skin infection Article Snippet: Slides were kept overnight at R, and images were taken using a Keyence fluorescence microscope (Keyence, BZ-X800/ BZ-X810) with a 40x or 100x objective. .. Naive CD4 + CD62L + T cells, purified from the spleens of uninfected C57BL/6N mice using the naive CD4 + T cell isolation kit (Miltenyi Biotec), were stimulated for 3 days with immobilized anti-CD3 (clone 145–2C11, BioXCell; culture wells coated with 5 μg/mL) in the presence of Th1- or Th2-skewed conditions (Th1: addition of IL-12 [R&D System, 419-ML, 5 ng/mL], Cell Isolation:Article Title: The metabolic program of inflammatory eosinophils accounts for chronic parasite-induced skin disease Article Snippet: Slides were kept overnight at R, and images were taken using a Keyence fluorescence microscope (Keyence, BZ-X800/ BZ-X810) with a 40x or 100x objective. .. Naïve CD4 + CD62L + T cells, purified from the spleens of uninfected C57BL/6N mice using the naïve CD4 + T cell isolation kit (Miltenyi Biotec), were stimulated for 3 days with immobilized anti-CD3 (clone 145–2C11, BioXCell; culture wells coated with 5 μg/mL) in the presence of Th1-skewed conditions, i.e. with addition of IL-12 (R&D System, 419-ML, 5 ng/μL), Article Title: Metabolically reprogrammed eosinophils impair T cell immunity and cause chronic skin infection. Article Snippet: Slides were kept overnight at R, and images were taken using a Keyence fluorescence © The Author(s) EMBO Molecular Medicine 15 microscope (Keyence, BZ-X800/ BZ-X810) with a 40x or 100x objective. .. Co-culture of sorted eosinophils with in vitro-generated Th1 or Th2 cells Naive CD4+CD62L+ T cells, purified from the spleens of uninfected C57BL/6N mice using the naive CD4+ T cell isolation kit (Miltenyi Biotec), were stimulated for 3 days with immobilized anti-CD3 (clone 145–2C11, BioXCell; culture wells coated with 5 μg/mL) in the presence of Th1- or Th2-skewed conditions (Th1: addition of IL-12 [R&D System, 419-ML, 5 ng/mL], Article Title: Metabolically reprogrammed eosinophils impair T cell immunity and cause chronic skin infection Article Snippet: Slides were kept overnight at R, and images were taken using a Keyence fluorescence microscope (Keyence, BZ-X800/ BZ-X810) with a 40x or 100x objective. .. Naive CD4 + CD62L + T cells, purified from the spleens of uninfected C57BL/6N mice using the naive CD4 + T cell isolation kit (Miltenyi Biotec), were stimulated for 3 days with immobilized anti-CD3 (clone 145–2C11, BioXCell; culture wells coated with 5 μg/mL) in the presence of Th1- or Th2-skewed conditions (Th1: addition of IL-12 [R&D System, 419-ML, 5 ng/mL], Control:Article Title: Compounds active towards nuclear receptors Article Snippet: The following modifications were made to the manufacturer's protocol: 1) Incubation with Biotin-Antibody Cocktail and Anti-Biotin MicroBeads was prolonged to 30 minutes, and 2) Cells were washed with 40 mL of Miltenyi buffer. .. Differentiation of Th17 cells in anti-CD3 (BD Pharmingen, 5 μg/ml) coated 96-well plates (400,000 cells/well, 160 μl RPMI 1640+10% Fetal Bovine Serum) containing 5 μg/ml anti-CD28 (BD Pharmingen), 10 ng/ml IL-2 (R&D Systems), 2.5 ng/ml TGFP-1 (R&D Systems), 20 ng/ml IL-1β (R&D Systems), 20 ng/ml IL-6 (R&D Systems), 30 ng/ml IL-23 (R&D Systems), 2.5 μg/ml Cell Differentiation:Article Title: Fludarabine Ameliorates Lupus Nephritis by Inhibiting T Cell Infiltration through STAT1 in R848-induced Mice Models Article Snippet: CD4+ T cells were purified from spleens of C57BL/6 mice using the CD4+ T cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions. .. For Th1 cell differentiation, purified CD4+ T cells were seeded at 1×106 cells/well and were activated with mouse T-activator CD3/CD28 DynabeadsTM (Invitrogen) and treated with 10 ng/mL IL-2, 10 ng/mL IL-12, 5 ng/mL IFNγ, 10 μg/mL |